Loewe Biochemica GmbH https://loewe-info.com/ Solutions for Plant Disease Diagnostics Mon, 22 Jun 2026 11:43:19 +0000 en-US hourly 1 https://wordpress.org/?v=7.0.4 https://loewe-info.com/wp-content/uploads/2022/11/cropped-TSWVAnthurie-32x32.jpg Loewe Biochemica GmbH https://loewe-info.com/ 32 32 Whitepaper Series Part 4 – How to ELISA data analysis – available now! https://loewe-info.com/whitepaper-series-part-4-available-now/ Mon, 22 Jun 2026 11:35:47 +0000 https://loewe-info.com/?p=13669 The post Whitepaper Series Part 4 – How to ELISA data analysis – available now! appeared first on Loewe Biochemica GmbH.

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ELISA in Plant Pathology: from Principle to Practice

A Whitepaper Series in 9 Chapters by LOEWE® Biochemica GmbH

Author: Dr. Simone Bode

 

Download Whitepaper Part 4
Chapter Overview

Part 1 – Fundamentals of ELISA Technology in Plant Pathology

Theoretical background, assay types, advantages/limitations

Part 2 – Sample Preparation and Extraction Techniques

Practical sample handling, extraction buffers, homogenization, troubleshooting

Part 3 – Performing the Assay: Step-by-Step Guide

Detailed walkthrough of plate setup, incubation, washing, substrate development

Part 4 – Data Analysis and Interpretation

Reading ELISA plates, understanding OD values, thresholds, and result validation

Part 5 – Troubleshooting and Quality Assurance

Common problems (background, cross-reactivity, low signal), QC strategies

Part 6 – Validation and Standardization

Method validation, reproducibility, inter-lab comparison, documentation

Part 7 – Advanced ELISA Formats and Innovations

Multiplex ELISA, automation, portable ELISA systems, digital readers

Part 8 – Field Applications and Case Studies

Practical use in seed health testing, quarantine diagnostics, greenhouse monitoring

Part 9 – Summary & Outlook

Key learnings, integration into diagnostic workflows, link to PCR or multiplex methods

The post Whitepaper Series Part 4 – How to ELISA data analysis – available now! appeared first on Loewe Biochemica GmbH.

]]> Whitepaper Series Part 3 – available now! https://loewe-info.com/whitepaper-series-part-3-available-now/ Wed, 13 May 2026 12:10:14 +0000 https://loewe-info.com/?p=13609 The post Whitepaper Series Part 3 – available now! appeared first on Loewe Biochemica GmbH.

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ELISA in Plant Pathology: from Principle to Practice

A Whitepaper Series in 9 Chapters by LOEWE® Biochemica GmbH

Author: Dr. Simone Bode

 

Download Whitepaper Part 3
Chapter Overview

Part 1 – Fundamentals of ELISA Technology in Plant Pathology

Theoretical background, assay types, advantages/limitations

Part 2 – Sample Preparation and Extraction Techniques

Practical sample handling, extraction buffers, homogenization, troubleshooting

Part 3 – Performing the Assay: Step-by-Step Guide

Detailed walkthrough of plate setup, incubation, washing, substrate development

Part 4 – Data Analysis and Interpretation

Reading ELISA plates, understanding OD values, thresholds, and result validation

Part 5 – Troubleshooting and Quality Assurance

Common problems (background, cross-reactivity, low signal), QC strategies

Part 6 – Validation and Standardization

Method validation, reproducibility, inter-lab comparison, documentation

Part 7 – Advanced ELISA Formats and Innovations

Multiplex ELISA, automation, portable ELISA systems, digital readers

Part 8 – Field Applications and Case Studies

Practical use in seed health testing, quarantine diagnostics, greenhouse monitoring

Part 9 – Summary & Outlook

Key learnings, integration into diagnostic workflows, link to PCR or multiplex methods

The post Whitepaper Series Part 3 – available now! appeared first on Loewe Biochemica GmbH.

]]> The new LOEWE Catalog 2026 has arrived! https://loewe-info.com/13514-2/ Thu, 19 Feb 2026 10:36:17 +0000 https://loewe-info.com/?p=13514 The post The new LOEWE Catalog 2026 has arrived! appeared first on Loewe Biochemica GmbH.

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Thank you for waiting:

our Catalog 2026 is finally here!

Take a first look!

The post The new LOEWE Catalog 2026 has arrived! appeared first on Loewe Biochemica GmbH.

]]> Discover our new Whitepaper Series for Plant ELISA! https://loewe-info.com/13351-2/ Fri, 13 Feb 2026 15:00:58 +0000 https://loewe-info.com/?p=13351 The post Discover our new Whitepaper Series for Plant ELISA! appeared first on Loewe Biochemica GmbH.

]]>

ELISA in Plant Pathology: from Principle to Practice

A Whitepaper Series in 9 Chapters by LOEWE® Biochemica GmbH

Author: Dr. Simone Bode

 

Download Whitepaper Part 2
Chapter Overview

Part 1 – Fundamentals of ELISA Technology in Plant Pathology

Theoretical background, assay types, advantages/limitations

Part 2 – Sample Preparation and Extraction Techniques

Practical sample handling, extraction buffers, homogenization, troubleshooting

Part 3 – Performing the Assay: Step-by-Step Guide

Detailed walkthrough of plate setup, incubation, washing, substrate development

Part 4 – Data Analysis and Interpretation

Reading ELISA plates, understanding OD values, thresholds, and result validation

Part 5 – Troubleshooting and Quality Assurance

Common problems (background, cross-reactivity, low signal), QC strategies

Part 6 – Validation and Standardization

Method validation, reproducibility, inter-lab comparison, documentation

Part 7 – Advanced ELISA Formats and Innovations

Multiplex ELISA, automation, portable ELISA systems, digital readers

Part 8 – Field Applications and Case Studies

Practical use in seed health testing, quarantine diagnostics, greenhouse monitoring

Part 9 – Summary & Outlook

Key learnings, integration into diagnostic workflows, link to PCR or multiplex methods

The post Discover our new Whitepaper Series for Plant ELISA! appeared first on Loewe Biochemica GmbH.

]]> Hop Viroid Detection by qPCR https://loewe-info.com/13030-2/ Tue, 30 Sep 2025 13:12:23 +0000 https://loewe-info.com/?p=13030 The post Hop Viroid Detection by qPCR appeared first on Loewe Biochemica GmbH.

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The post Hop Viroid Detection by qPCR appeared first on Loewe Biochemica GmbH.

]]> NEW: REALTIME KITS for Cereal Virus Detection! https://loewe-info.com/new-realtime-kits-for-cereal-virus-detection/ Wed, 26 Mar 2025 10:58:12 +0000 https://loewe-info.com/?p=11764 The post NEW: REALTIME KITS for Cereal Virus Detection! appeared first on Loewe Biochemica GmbH.

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Detection of the cereal viruses BSMV, WSMV and WDV with LOEWE®REALTIME 

Cat. No. 06014C/100

Barley Stripe Mosaic Virus (BSMV)

BSMV infects barley (Hordeum vulgare), wheat (Triticum aestivum), oats (Avena sativa), and other grasses, leading to stripe or mosaic patterns on leaves, which appear yellow or greenish, stunted growth, leaf deformations, and reduced yield.
BSMV can cause significant yield losses in areas with high cereal production, especially under heavy infestation pressure.
Our realtime assay can detect as low as 1 fg of total RNA from infected leaves.

Cat. No. 06048C/100

Wheat Streak Mosaic Virus (WSMV)

WSMV primarily affects wheat but can also infect other cereals like barley, rye, and various grasses.
It causes yellow mosaic and streak patterns on leaves. In severe cases, plants may become stunted, and yields significantly decrease.
WSMV causes major damage in wheat fields, especially in North America and arid regions, as it is transmitted by wheat curl mites.
Our realtime assay can detect as low as 0.1 pg of total RNA from infected leaves.

Cat. No. 06082C/100

Wheat Dwarf Virus (WDV)

WDV primarily infects wheat but can also affect rye, barley, and some other grasses.
It causes stunting, pale or yellowing leaves, and plant dwarfism. It can reduce both the number and size of grains.
 WDV is particularly significant in Europe and Asia and can lead to substantial yield losses in wheat fields, especially when transmitted by insect vectors like aphids.
Our realtime assay can detect as low as 0.1 pg of total RNA from infected leaves.

 

Our REALTIME Kits feature:

Reaction set-up and shipment without dry ice!

One-tube One-step setup!

Internal Control reaction included to monitor successful extraction of plant RNA

Sensitive and specific detection of CBCVd, HLVd or HSVd

ROX included as passive reference dye, if your cycler requires normalization of the fluorescent signal

The post NEW: REALTIME KITS for Cereal Virus Detection! appeared first on Loewe Biochemica GmbH.

]]> NEW: Viroid Detection! https://loewe-info.com/new-launch-of-three-new-diagnostic-kits-2/ Wed, 23 Oct 2024 08:15:19 +0000 https://loewe-info.com/?p=11571 The post NEW: Viroid Detection! appeared first on Loewe Biochemica GmbH.

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Detection of Plant Viroids with LOEWE®REALTIME 

Hop Latent Viroid

HLVd infection in hop often remains asymptomatic, but can cause yield loss and changes in bitter acid contents and terpenes. On the other hand, HLVd infection of hemp and cannabis causes Dudding disease, which has become the most devastating disease in cannabis-growing areas.

Lit.: Adkar-Purushothama et al. 2023

Analytical data for HLVd:

LOD: 1 pg of RNA from HLVd infected RNA, 41 copies  of synthetic DNA

Diagnostic sensitivity: 100 %

Analytical specificity: 100 %

Citrus Bark Cracking Viroid

CBCVd is a minor pathogen on citrus species, which induces reduced growth and canopy volume in infected plants, as well as yield. However, CBCVd is a severe pathogen in hop, resulting in plant stunting, leaf yellowing and curling etc. As consequence plants die within 3-5 years after infection!

Lit.: EPPO Datasheet: Citrus bark cracking viroid

Analytical data for CBCVd:

LOD: 100 fg RNA of CBVd infected RNA, 8 copies  of synthetic DNA

Diagnostic sensitivity: 100 %

Analytical specificity: 100 %

Hop Stunt Viroid
HSVd was first identified in hop plants causing hop stunt disease. Because of the dwarf shape and stunted growth of infected plants with reduced cones per bine and altered alpha acids content HSVd has a high economic impact for hop growers. HSVd has a wide natural host range and can cause diseases in citrus plants, stone fruits or cucumbers.

Lit.: Hataya, T. et al. 2017

Analytical data for HSVd:

LOD: 10 pg of RNA from HSVd infected RNA,  29 copies  of synthetic DNA

Diagnostic sensitivity: 100 %

Analytical specificity: 100 %

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Our REALTIME Kits feature:

Reaction set-up and shipment without dry ice!

One-tube One-step setup!

Internal Control reaction included to monitor successful extraction of plant RNA

Sensitive and specific detection of CBCVd, HLVd or HSVd

ROX included as passive reference dye, if your cycler requires normalization of the fluorescent signal

The post NEW: Viroid Detection! appeared first on Loewe Biochemica GmbH.

]]> NEW: Launch of three new diagnostic kits https://loewe-info.com/new-launch-of-three-new-diagnostic-kits/ Wed, 20 Mar 2024 07:40:06 +0000 https://loewe-info.com/?p=11120 The post NEW: Launch of three new diagnostic kits appeared first on Loewe Biochemica GmbH.

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Launch of three new diagnostic kits:

 

LOEWE®REALTIME complete reaction kit –  Fast and highly sensitive

  • Most sensitive assay for detection 
  • One-step one-tube assay
  • Probe-based with flourescence detection on the FAM™-channel
  • ships at room temperature!

RNA-PCR complete reaction kit 

Parietaria Mottle Virus (PMoV) is considered as an emerging plant pathogen in the Mediterranean region. Mixed infections with other tomato and pepper infecting viruses have been reported (as ToBRFV, TWSV, CMV), resulting in an increased appearance of symptoms.
PMoV specific primers ensure specific and sensitive end-point PCR detection.
  • lntended use: testing  of  fresh,  frozen  or  dried  leaf  and fruit  material from tomato and pepper

LOEWE®FAST Lateral Flow Assay – Fast – on the spot

  • Fast and easy identificaton of of Dickeya chrysanthemi on agar culturest!
  • Test results are available in minutes without the need of a laboratory

The post NEW: Launch of three new diagnostic kits appeared first on Loewe Biochemica GmbH.

]]> New Catalog 2024 https://loewe-info.com/11268-2/ Tue, 09 Jan 2024 15:19:15 +0000 https://loewe-info.com/?p=11268 The post New Catalog 2024 appeared first on Loewe Biochemica GmbH.

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Contact Us for Your 2024 PDF Catalog

4 + 13 =

Download our Catalog here!

The post New Catalog 2024 appeared first on Loewe Biochemica GmbH.

]]> NEW: Launch of three new diagnostic kits https://loewe-info.com/launch-of-three-new-diagnostic-kits/ Fri, 11 Aug 2023 09:41:15 +0000 https://loewe-info.com/?p=11018 The post NEW: Launch of three new diagnostic kits appeared first on Loewe Biochemica GmbH.

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Launch of three new diagnostic kits:

 

LOEWE®REALTIME complete reaction kit –  Fast and highly sensitive

  • Most sensitive assay for detection 
  • One-step one-tube assay
  • Probe-based with flourescence detection on the FAM™-channel
  • ships at room temperature!

RNA-PCR complete reaction kit 

Parietaria Mottle Virus (PMoV) is considered as an emerging plant pathogen in the Mediterranean region. Mixed infections with other tomato and pepper infecting viruses have been reported (as ToBRFV, TWSV, CMV), resulting in an increased appearance of symptoms.
PMoV specific primers ensure specific and sensitive end-point PCR detection.
  • lntended use: testing  of  fresh,  frozen  or  dried  leaf  and fruit  material from tomato and pepper

LOEWE®FAST Lateral Flow Assay – Fast – on the spot

  • Fast and easy identificaton of of Dickeya chrysanthemi on agar culturest!
  • Test results are available in minutes without the need of a laboratory

The post NEW: Launch of three new diagnostic kits appeared first on Loewe Biochemica GmbH.

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