Detection of Plant Viroids with LOEWE®REALTIME
Hop Latent Viroid
HLVd infection in hop often remains asymptomatic, but can cause yield loss and changes in bitter acid contents and terpenes. On the other hand, HLVd infection of hemp and cannabis causes Dudding disease, which has become the most devastating disease in cannabis-growing areas.
Lit.: Adkar-Purushothama et al. 2023
Analytical data for HLVd:
LOD: 1 pg of RNA from HLVd infected RNA, 41 copies of synthetic DNA
Diagnostic sensitivity: 100 %
Analytical specificity: 100 %
Citrus Bark Cracking Viroid
CBCVd is a minor pathogen on citrus species, which induces reduced growth and canopy volume in infected plants, as well as yield. However, CBCVd is a severe pathogen in hop, resulting in plant stunting, leaf yellowing and curling etc. As consequence plants die within 3-5 years after infection!
Lit.: EPPO Datasheet: Citrus bark cracking viroid
Analytical data for CBCVd:
LOD: 100 fg RNA of CBVd infected RNA, 8 copies of synthetic DNA
Diagnostic sensitivity: 100 %
Analytical specificity: 100 %
Hop Stunt Viroid
HSVd was first identified in hop plants causing hop stunt disease. Because of the dwarf shape and stunted growth of infected plants with reduced cones per bine and altered alpha acids content HSVd has a high economic impact for hop growers. HSVd has a wide natural host range and can cause diseases in citrus plants, stone fruits or cucumbers.
Lit.: Hataya, T. et al. 2017
Analytical data for HSVd:
LOD: 10 pg of RNA from HSVd infected RNA, 29 copies of synthetic DNA
Diagnostic sensitivity: 100 %
Analytical specificity: 100 %
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